![]() ![]() 2001) or evaluate vaccine targets for AlHV-1/OvHV-2 chimeric viruses (Cunha et al. By using this on primers of the gB gene, it is possible to investigate the geographical localisation amongst the sequence variations of OvHV-2 strains (Dunowska et al. Glycoprotein B plays a role in virus entry to a host organism and spread between cells. The glycoprotein B (gB) gene of OvHV-2 is the most conserved herpes virus protein, encoded by ORF 8 (Pereira 1994). The tegument gene OvHV-2 is encoded by open reading frame (ORF) 33 and ORF 75 and used for the purpose of detection of OvHV-2 DNA and phylogenetic analysis based on sequence data (Baxter et al. Famous findings have described high fever, generalised lymphadenopathy, keratoconjunctivitis, corneal oedema and opacity, but these symptoms are not always evident (Plowright 1990). Recently, new forms, neurological syndrome and systemic necrotising vasculitis, have been described that make determining clinical pictures and diagnosing the disease difficult (Martins et al. The clinicopathological findings of MCF in affected animals are not clear about differential diagnoses. To date, more than 10 identified causative viruses have been associated with this infection in domestic and wildlife ruminants (Crawford et al. Two causative agents for MCF are predominantly described, one of them carried by sheep infected with ovine herpesvirus 2 (OvHV-2) (called sheep-associated MCF) and the other carried by wildebeest ( Connochaetes species) containing alcelaphine herpesvirus 1 (AlHV-1) (called wildebeest-associated form) (Roizman 1992 Russel, Stewart & Haig 2009). Known as a fatal infection, malignant catarrhal fever (MCF) affects susceptible cattle, wild ruminants and pigs (Plowright, Ferris & Scott 1960). Keywords: malignant catarrhal fever tegument gene gB gene OvHV-2 Turkey. Although the obtained sequences were genetically different from each other, it was found that genetic variations were limited. The results of nucleotide sequencing of polymerase chain reaction (PCR) products indicated presence of sheep-associated form for MCF infection in Turkey. Tegument gene (422 base pairs) and glycoprotein B (gB) gene (2800 base pairs) amplicons of OvHV-2 genome were used for understanding of epidemiology of malignant catarrhal fever (MCF) infection in Turkey. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. In this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution,Īnd reproduction in any medium, provided the original work is properly cited. Oğuzoğlu, Seçkin Salar, Ece Adıgüzel, Cansu Demirden, Onur Ülgenalp ĭetection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2 & Ülgenalp, O., 2020, ‘Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2’, Onderstepoort Journal of Veterinary Research 87(1), a1886. ![]()
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